Research Article | Volume: 4, Issue: 8, August, 2014

Phenol content and antioxidant activity of different young and adult plant parts of tobacco from Tunisia, dried at 40 and 70 °C

Soumeya Ben Nasr Smail Aazza Wissem Mnif Maria Miguel   

Open Access   

Published:  Aug 27, 2014

DOI: 10.7324/JAPS.2014.40805
Abstract

Different parts of tobacco plants were dried at 40 and 70 ºC. Some of them were also dried at room temperature. Dried plant material was extracted by sonication for obtaining hydro-alcoholic extracts (70%). Total phenol and total flavonoids were determined as well as antioxidant activities which were evaluated through different methods (capacity for scavenging DPPH, ABTS, superoxide and hydroxyl radicals; capacity for preventing lipid peroxidation using egg yolk as substrate; and reducing power). In young and adult plants, leaves generally had higher amounts of phenols (14.46-23.05 mg GAE g-1) than the remaining parts of the plant, independent on the temperature used. Generally, roots had lower amounts of phenols (1.56-4.63 mg GAE g-1). Leaves and flower had significantly higher concentrations of flavonoids (3.08-4.17 mg QE g-1 and 1.17-2.12 mg QE g-1, respectively) than the remaining parts. The antioxidant activity was generally higher in leaf extracts, although stalk ones had also a good capacity for scavenging hydroxyl radicals. Generally, young plants had the best capacity for scavenging DPPH and hydroxyl free radicals which may be related with the phenol content. Concerning drying temperatures, the results were not conclusive.


Keyword:     Free radical scavenging lipid prevention developmental stage Nicotiana tabacum.


Citation:

Soumeya Nasr, Smail Aazza, Wissem Mnif, Maria Miguel. Phenol content and antioxidant activity of different young and adult plant parts of tobacco from Tunisia, dried at 40 and 70 ºC. J App Pharm Sci, 2014; 4 (08): 023-031.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

HTML Full Text

Reference

Article Metrics
647 Views 32 Downloads 679 Total

Year

Month

Related Search

By author names