Research Article | Volume: 4, Issue: 8, August, 2014

Evaluation of in-vitro apoptosis induction, cytotoxic activity of Hymenodictyon excelsum (Roxb) Wall in Dalton’s lymphoma ascites (DLA) and Lung fibroblast - Mouse L929 cell lines

Kalathil Khairunnisa and Dhananjayan Karthik   

Open Access   

Published:  Aug 27, 2014

DOI: 10.7324/JAPS.2014.40803
Abstract

The purpose of present study was to investigate the methanolic bark extract of Hymenodictyon excelsum (Roxb) Wall for its apoptosis, cytotoxic activities. Induction of apoptosis was carried out on L-929 cell line. The cytotoxic activity was evaluated by MTT assay against lung fibroblast (L-929) cell line and trypan blue dye exclusion assay on dalton’s lymphoma ascites (DLA) cells. Morphological changes and DNA fragmentation were found upon incubation with extract. The extract was found to be cytotoxic towards L-929 cells in 72 h MTT assay and concentration required for 50% cell death was 3.85µg/ml. Thus from the present investigation it can be concluded that the methanolic bark extract of Hymenodictyon excelsum (Roxb) exhibited apoptosis induction and cytotoxic activities.


Keyword:     Apoptosis cytotoxicity Hymenodictyon excelsum MTT assay Neoplasia.


Citation:

Kalathil Khairunnisa, Dhananjayan Karthik. Evaluation of in-vitro apoptosis induction, cytotoxic activity of Hymenodictyon excelsum (Roxb) Wall in Dalton’s lymphoma ascites (DLA) and Lung fibroblast - Mouse L929 cell lines. J App Pharm Sci, 2014; 4 (08): 011-017.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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