Research Article | Volume: 4, Issue: 7, July, 2014

A rapid and simple high performance thin layer chromatographic method for simultaneous analysis of β-sitosterol-D-glucoside, gallic acid, withaferin A and withanolide A in Ashvagandharishta

Prashant D Bhondave Sachin E Potawale Satish Y Gabhe Abhay M Harsulkar   

Open Access   

Published:  Jul 28, 2014

DOI: 10.7324/JAPS.2014.40714
Abstract

A new, simple, precise, accurate and robust high‐performance thin‐layer chromatographic (HPTLC) method has been developed for simultaneous estimation of β-sitosterol-D-glucoside, gallic acid, withaferin A and withanolide A in an Ayurvedic formulation, Ashvagandharishta. The plates were developed in mobile phase consisting of toulene: ethyl acetate: formic acid: methanol (6:3:0.1:0.6, v/v/v/v). The retention factors of β-sitosterol-D-glucoside, gallic acid, withaferin A and withanolide A were 0.18 ± 0.01, 0.26 ± 0.02, 0.37 ± 0.02 and 0.57 ± 0.02, respectively. Linearity was in the range of 50‐400 ng/band for β-sitosterol‐D‐glucoside, 150-500 ng/band for gallic acid, 50‐450 ng/band for withaferin A and 50-500 ng/band for withanolide A. Accuracy study showed recoveries of 99.04 - 102.67, 98.77 - 101.96, 99.64 - 102.67 and 100.07 - 101.96 % for β-sitosterol-D-glucoside, gallic acid, withaferin A and withanolide A, respectively. The results obtained in validation assays indicated accuracy and reliability of HPTLC method for simultaneous quantification of β‐sitosterol‐D‐glucoside, gallic acid, withaferin A and withanolide A in Ashvagandharishta. The percent content of β-sitosterol-D-glucoside, gallic acid, withaferin A and withanolide A in Ashvagandharishta were found to be 0.012, 0.064, 0.016 and 0.312 %, respectively.


Keyword:     Ashvagandharishta Withanoloids HPTLC Validation.


Citation:

Prashant D Bhondave, Sachin E Potawale, Satish Y Gabhe, Abhay M Harsulkar. Rapid and Simple High Performance Thin Layer Chromatographic Method for Simultaneous Analysis of βsitosterol-D-glucoside, gallic acid, withaferin A and withanolide A in Ashvagandharishta. J App Pharm Sci, 2014; 4 (07): 082-087.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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