Research Article | Volume: 4, Issue: 7, July, 2014

Functional analysis of ATP-binding cassette transporter of Streptomyces coelicolor

Tu Nguyen and Huu Nguyen   

Open Access   

Published:  Jul 28, 2014

DOI: 10.7324/JAPS.2014.40709
Abstract

ATP- binding cassette (ABC) transporters are characterized as multi drug resistant transporters utilizing ATP hydrolysis. The SCO5113 (GeneID: 1100554) encoding a multidrug resistance ABC transporter in Streptomyces coelicolor was expressed in E.coli BL21 (DE3) plysS, using pET 21 a(+). The expression of his- tag fused SCO5113 at 65 kDa under 0.1 M IPTG in 6 h was detected on SDS-PAGE after purification using affinity chromatography. The E. coli BL21 (DE3) plysS harboring SCO5113 was used to test for the antibiotic resistance, using disc diffussion method. The results showed the resistance of E. coli BL21 (DE3) plysS harboring SCO5113 to five kinds of tested antibiotics such as cephalothin (1mg/ml), kanamycin (1mg/ml), ampicillin (10mg/ml), erythromycin (10mg/ml), and chloramphenicol (1mg/ml). The study reported the function of the SCO5113 gene of Streptomyces coelicolor A3(2).


Keyword:     ABC transporter antibiotic resistance functional analysis Streptomyces coelicolor.


Citation:

Tu Nguyen, Huu Nguyen. Functional Analysis of Atp-Binding Cassette Transporter of Streptomyces Coelicolor. J App Pharm Sci, 2014; 4 (07): 050-053.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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