ATP- binding cassette (ABC) transporters are characterized as multi drug resistant transporters utilizing ATP hydrolysis. The SCO5113 (GeneID: 1100554) encoding a multidrug resistance ABC transporter in Streptomyces coelicolor was expressed in E.coli BL21 (DE3) plysS, using pET 21 a(+). The expression of his- tag fused SCO5113 at 65 kDa under 0.1 M IPTG in 6 h was detected on SDS-PAGE after purification using affinity chromatography. The E. coli BL21 (DE3) plysS harboring SCO5113 was used to test for the antibiotic resistance, using disc diffussion method. The results showed the resistance of E. coli BL21 (DE3) plysS harboring SCO5113 to five kinds of tested antibiotics such as cephalothin (1mg/ml), kanamycin (1mg/ml), ampicillin (10mg/ml), erythromycin (10mg/ml), and chloramphenicol (1mg/ml). The study reported the function of the SCO5113 gene of Streptomyces coelicolor A3(2).
Tu Nguyen, Huu Nguyen. Functional Analysis of Atp-Binding Cassette Transporter of Streptomyces Coelicolor. J App Pharm Sci, 2014; 4 (07): 050-053.
534 Absract views 5 PDF Downloads 539 Total views
Prevalence, antibiotic and oil resistance pattern of some bacterial isolates from burns
Studies on the Prodigiosin Production from Streptomyces coelicolor in Liquid Media by Using Heated Lactobacillus rhamnosus