Research Article | Volume: 4, Issue: 7, July, 2014

In vitro study on the efficacy of zinc oxide and titanium dioxide nanoparticles against metallo beta-lactamase and biofilm producing Pseudomonas aeruginosa

Mary Grace Vincent Neetu Peedikayil John Narayanan PM Vani C and Sevanan Murugan   

Open Access   

Published:  Jul 28, 2014

DOI: 10.7324/JAPS.2014.40707

Pseudomonas aeruginosa contributes to a multitude of infections exhibiting intrinsic resistance to numerous antibiotics. Metallo beta-lactamase and biofilm production is the most worrisome resistant mechanisms observed in P. aeruginosa. Emergence of antimicrobial resistance by pathogenic bacteria is a major health problem in recent years. Zinc oxide and titanium dioxide nanoparticles comprises of well-known inhibitory and bactericidal effects. The present study is designed to determine the efficacy of zinc oxide and titanium dioxide nanoparticles against metallo beta-lactamase and biofilm producing P. aeruginosa. A total of 51 non-repetitive P. aeruginosa isolates were obtained from Bioline laboratory, Coimbatore. Biofilm and metallo beta-lactamase production was tested by combined disc test and tissue culture plate method. Commercially available zinc and titanium dioxide nanoparticles were obtained and tested against two metallo beta-lactamase and biofilm producing isolates. Both the nanoparticles showed appreciable activity at all tested concentrations. Thus, it is concluded that ZnO and TiO2 nanoparticles may serve as a promising antibacterial agents in coming years.

Keyword:     Pseudomonas aeruginosa Biofilms Metallo beta-lactamase Zinc oxide nanoparticles Titanium dioxide nanoparticles Antimicrobial activity.


Mary Grace Vincent, Neetu Peedikayil John, Narayanan PM, Vani C and Sevanan Murugan. Invitro study on the efficacy of Zinc Oxide and Titanium Dioxide nanoparticles against Metallo betalactamase and Biofilm producing Pseudomonas aeruginosa. J App Pharm Sci, 2014; 4 (07): 041-046.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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