Research Article | Volume: 4, Issue: 6, June, 2014

Identification, Isolation and Characterization of IGF Binding Proteins as a Secretable Proteins of Human Endometrial Stromal Cell Culture

Gamal A. M. Singer Thomas Strowitzki Jihan Hussein Fatma Oraby   

Open Access   

Published:  Jun 28, 2014

DOI: 10.7324/JAPS.2014.40601
Abstract

IGF-1, a high potent mitogenic factor with a dual function is believed to participate with female steroids and other growth factors to prepare endometrium receptivity for successful embryo implantation and further development. Stromal cells forms the functional receptive layer of endometrium structure. Analysis of the conditioned/defined medium of pure cultured human endometrial stromal cells have revealed that stromal cells in a monolayer culture secrete and produce three different types of proteins in a varying amounts. These proteins exhibit a high specificity as well as a high binding affinity for the radiolabeled IGF-1 peptide. The molecular weights of these proteins have been determined by SDS-Page electrophoresis after cross-linking with the radiolegand IGF-1 and then detected with autoradiography. By comparison to the migration of high molecular weights protein markers, these proteins have been identified to correspond to the IGFBP-1 (31 KDa), IGFBP-2 (36 KDa) and IGFBP-3 (45 KDa). The secretion of these binding proteins (IGFBP-1, -2, -3) by endometrial stromal cells may support the view of their biological importance in controlling the delivery and bioavailability of the high mitogen IGF-1 peptide to their nominative type-1 IGF-receptor on cell surface, thereby modulating its action. It seems likely that these IGFBPs may play a key role in switching on/off IGF-1 peptide action , thereby avoiding the uncontrolled proliferation effect of the IGF-1 that favor endometrium cancer development.


Keyword:     IGFBPs Cell culture Endometrial Stromal cells.


Citation:

Gamal A.M. Singer, Thomas Strowitzki, Jihan Hussein, Fatma Oraby. Identification, Isolation and Characterization of IGF Binding Proteins as a Secretable Proteins of Human Endometrial Stromal Cell Culture. J App Pharm Sci, 2014; 4 (06): 001-008.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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