Research Article | Volume: 4, Issue: 5, May, 2014

Estimation of ursolic acid from Urtica dioica L. using validated HPTLC method

Sunita Shailajan Harshada Hande Dipti Singh Bhavesh Tiwari   

Open Access   

Published:  May 27, 2014

DOI: 10.7324/JAPS.2014.40517

Urtica dioica L. (Urticaceae, leaves) is commonly used in traditional systems of medicine for the treatment of a wide range of disorders. The present work emphasizes on a validated HPTLC method for estimation of ursolic acid from U. dioica leaves and its available formulation. Chromatographic separation was achieved on silica gel 60 F254 TLC plate with toluene: ethyl acetate: formic acid (7:3:0.1, v/v/v) as a mobile phase. Detection of ursolic acid was carried out by derivatizing the plate with Liebermann Burchard reagent at 110°C for 10 min. Camag TLC scanner 4 equipped with winCATS software was used for densitometric scanning at 366 nm. The accuracy of the method was checked by conducting various validation parameters according to ICH guidelines. The method was found applicable to evaluate the impact of regional variation on ursolic acid content in U. dioica leaves. The research also highlights estimation of ursolic acid from a marketed herbal formulation of U. dioica leaves. The described HPTLC method was found useful for quantitation of bioactive marker ursolic acid and can be used as a routine quality control tool for the assessment of botanicals.

Keyword:     Geographical variation HPTLC leaves ursolic acid Urtica dioica.


Sunita Shailajan, Harshada Hande, Dipti Singh, Bhavesh Tiwari., Estimation of ursolic acid from Urtica dioica L. using validated HPTLC method. J App Pharm Sci, 2014; 4 (05): 092-095.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

HTML Full Text


Article Metrics

660 Absract views 8 PDF Downloads 668 Total views

   Abstract      Pdf Download

Related Search

By author names

Citiaion Alert By Google Scholar

Name Required
Email Required Invalid Email Address

Comment required