Research Article | Volume: 4, Issue: 5, May, 2014

Simultaneous estimation of Cefpodoxime proxetil and Ofloxacin In tablet dosage form using RP-HPLC

Annadi Chiranjeevi and Medidi Srinivas   

Open Access   

Published:  May 27, 2014

DOI: 10.7324/JAPS.2014.40508

A simple, rapid and precise reverse phase liquid chromatographic (RP-HPLC) method was developed and subsequently validated for simultaneous estimation of Cefpodoxime proxetil and Ofloxacin in combined fixed dose oral formulation. The analysis was carried out using X-terra C8 (4.6 x 250mm, 5µm, Make: ACE), pre-packed column. The separation was carried out using a mobile phase containing a 0.25%v/v triethyl amine buffer of pH 3.5 and acetonitrile (30:70 v/v), was pumped at a flow rate of 1.2 ml/min with UV-detector and PDA detection at 227 nm. Both the drugs were well resolved on the stationary phase and the retention times were around 2.747 minute for Cefpodoxime proxetil and 2.076 minute for Ofloxacin. The method was validated and shown to be linear for Cefpodoxime proxetil and Ofloxacin. The correlation coefficients for Cefpodoxime proxetil and Ofloxacin are 0.998 and 0.999 respectively. The relative standard deviations for five replicate measurements in two sets of each drug in the tablets is always less than 2% and mean % error of active recovery not more than ±1.5%. The method was validated for precision and accuracy. The developed method could be applied for routine analysis of Cefpodoxime proxetil and Ofloxacin in tablet dosage form without any interference of excipients.

Keyword:     Cefpodoxime proxetil Ofloxacin RP-HPLC method development and Validation.


Annadi Chiranjeevi, Medidi Srinivas., Simultaneous estimation of Cefpodoxime proxetil and Ofloxacin In tablet dosage form using RP-HPLC. J App Pharm Sci, 2014; 4 (05): 046-050.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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