Research Article | Volume: 4, Issue: 3, March, 2014

In-vitro antidermatophytic and preliminary phytochemical studies of petroleum ether and Inter-polar methanolic leaf extracts of Thevetia nerrifolia

Shivakumar Singh P and Vidyasagar G M   

Open Access   

Published:  Feb 27, 2014

DOI: 10.7324/JAPS.2014.40316
Abstract

Antidermatophytic activity (Agar well diffusion technique) of petroleum ether and 98% methanolic leaf extracts of Thevetia nerrifolia (Apocynaceae) was evaluated against mycotic fungi namely, Trichophyton rubrum, Trichophyton tonsurans, Trichophyton mentagrophytes, Microsporum gypseum, dimorphic fungi such as, Candida albicans and pathogenic bacteria like, Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Brevibacillus spp. The significant antidermatophytic activity was shown by 98% methanolic extract, which inhibited all the test fungi and bacteria with time and dose dependent activity. The 98% methanolic extract retarded the growth of all the organisms at 40 mg/ml up to three weeks and beyond. The MIC’s, MFC’s and MBC’s were determined against all the test strains. Preliminary Phytochemical tests were carried out using both the crude extracts. This study provides basis for the isolation and purification of anti-dermatophytic inter polar compounds from the leaves of T. nerrifolia.


Keyword:     Antidermatophytic Activity 98% Methanolic leaf extract Thevetia nerrifolia Inter-polar compounds.


Citation:

Shivakumar Singh P and Vidyasagar G M. In-vitro antidermatophytic and preliminary phytochemical studies of petroleum ether and Inter-polar methanolic leaf extracts of Thevetia nerrifolia. J App Pharm Sci, 2014; 4 (03): 081-085.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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