Published:  Jan 30, 2014DOI: 10.7324/JAPS.2014.40126
Development of anti-melanogenic agents from natural sources has long been the research project of our laboratory. In this study, ethyl acetate extracts of Oreocnide fruticosa (Gaudich.) Hand.-Mazz. branches (OBE) were examined for their melanin synthesis inhibitory activities using B16 melanoma cells. Our results indicated that OBE down-regulates melanin production in a dose-dependent pattern. To clarify the target of OBE action in melanogenesis, we performed Western blotting for the key melanogenic enzymes; tyrosinase, tyrosinase related protein-1 (TRP-1) and TRP-2. The results showed that OBE efficiently inhibited tyrosinase, TRP-1 and TRP-2 in a dose-dependent manner. Therefore, OBE is a good candidate for the further study including identification of the effective chemical constituents as well as their working mechanism for the application of whitening agent in the human skin.
Jung Hwan Han, Sang-Hee Byeon, Chang-Gu Hyun and Nam Ho Lee. Melanogenesis Inhibitory Activity in the Extracts of Oreocnide fruticosa (Gaudich.) Hand.-Mazz. Branches. J App Pharm Sci, 2014; 4 (01): 166-169.
283 Absract views 5 PDF Downloads 288 Total views