Research Article | Volume: 4, Issue: 1, January, 2014

In vitro Antioxidant, Lipoxygenase and Xanthine Oxidase inhibitory activity of fractions and macerate from Pandiaka angustifolia (vahl) Hepper

A.M.E. Thiombiano,  Hilou Adama,  Bangou Mindiédiba Jean,  Bagora Bayala, Ouattara Nabèrè,  Guenné Samson,  Meda Nâg-Tiero Roland,  Compaoré Moussa,  Kiendrébeogo Martin,  Millogo F. Jeanne and Nacoulma O. Germaine   
A.M.E. Thiombiano1, Hilou Adama1, Bangou Mindiédiba Jean, Bagora Bayala3,Ouattara Nabèrè1, Guenné Samson1, Meda Nâg-Tiero Roland1, Compaoré Moussa1, Kiendrébeogo Martin1, Millogo F. Jeanne2 and Nacoulma O. Germaine1

1Laboratory of Biochemistry and Applied Chemistry (LABIOCA), UFR/SVT, 09 BP 848 Ouagadougou 09, University of Ouagadougou (Burkina Faso).

2Laboratory of Biology and plants Ecology, UFR/SVT, 09 BP 848 Ouagadougou 09, University of Ouagadougou (Burkina Faso).

3Centre de Recherche Biomoléculaire Pietro annogoni,Saint Camille-CERBA/ LABIOGENE- Ouagadougou, Université de Ouagadougou,03 BP 7021 ouagadougou 03 (Burkina Faso).

Open Access   

Published:  Jan 30, 2014

DOI: 10.7324/JAPS.2014.40102
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Abstract

Pandiaka angustifolia (Vahl) Hepper, a species belonging to Amaranthaceae’s family is used in traditional medicine of Burkina Faso for the treatment of several diseases. The aim of this study was to evaluate the phenolics and flavonoids content, the antioxidant capacity, the lipoxygenase and xanthine oxidase inhibition potentials of Pandiaka angustifolia hydroacetonic macerate and fractions. Assay revealed that the Dichloromethane Fraction (DCMF) possess the best inhibitory activity of lipoxygenase, a key enzyme involved in inflammatory process with 83.84±1.89% of inhibition, as regards Xanthine Oxidase (XO) inhibiting percentage, n-hexane fraction (n-HF) exhibited the highest percentage of inhibition with 76.22 ± 4.78% at a concentration of 100µg/ml. Phenolics and flavonoids content in fractions and hydroacetonic macerate evaluation showed that hydroacetonic macerate possess the highest rate in total phenolic and the Dichloromethane fraction (DCMF) the highest rate in flavonoids content. This study showed that the apolar fractions of P. angustifolia is a potential natural source for the treatment of oxidative stress and inflammatory related diseases.


Keyword:     Pandiaka angustifolia antioxydant activity LOX XO phenolic content.

Citation:

A.M.E. Thiombiano , Hilou Adama, Bangou Mindiédiba Jean, Bagora Bayala,Ouattara Nabèrè, Guenné Samson, Meda NâgTiero Roland, Compaoré Moussa, Kiendrébeogo Martin, Millogo F. Jeanne and Nacoulma O. Germaine. In vitro Antioxidant, Lipoxygenase and Xanthine Oxidase inhibitory activity of fractions and macerate from Pandiaka angustifolia (vahl) Hepper. J App Pharm Sci, 2014; 4 (01): 009-013.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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