Research Article | Volume: 3, Issue: 12, December, 2013

Antioxidant and anti-proliferative activities of Sabah Ruellia tuberosa

Bo Eng Cheonga Mohd. Zulkarnain Waslimb Fui Fui Lema Peik Lin Teoha   

Open Access   

Published:  Dec 30, 2013

DOI: 10.7324/JAPS.2013.31204
Abstract

The study was carried out to evaluate the total phenolic constituents, antioxidant and anti-proliferative activities of Sabah Ruellia tuberosa. The total phenolic and flavonoid contents of the plant extracts were determined by using Folin-Ciocalteau and aluminium chloride colorimetric assays, respectively. The antioxidant activity of the plant extracts was evaluated using DPPH free radical scavenging assay while the anti-proliferative activity was evaluated using MTT assay against the human breast cancer (MCF-7) and cervical cancer (HeLa) cell lines. The methanol leaf extract was found to possess the highest total phenolic content (82.67 ± 2.09 mg GAE/g) while the ethyl acetate leaf extract was found to possess the highest total flavonoid content (152.77 ± 4.68 mg Cat/g). The ethyl acetate leaf possessed the highest radical scavenging activity, with IC50 of 720 μg/ml. Meanwhile, the methanol stem extract showed the highest anti-proliferative activity against MCF-7 cancer cells, with IC50 of 22 μg/ml but none of the extracts exhibited strong anti-proliferative activity against the HeLa cancer cell lines. Significant correlation was found between the total phenolic/flavonoid contents with the total antioxidant activity while weak correlation was found between the total phenolic/flavonoid contents with the inhibition of MCF-7 cell proliferation. Our findings indicate that Sabah Ruellia tuberosa could be a potential source for natural antioxidant as well as chemo-preventive agent against breast cancer in future. Thus, further isolation and characterization of the respective bioactive compounds from the plants are necessary.


Keyword:     Ruellia tuberosaantioxidant activity anti-proliferative activity.


Citation:

Bo Eng Cheong, Mohd. Zulkarnain Waslim, Fui Fui Lem, Peik Lin Teoh. Antioxidant and anti-proliferative activities of Sabah Ruellia tuberose. J App Pharm Sci, 2013; 3 (12): 020-024.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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