Research Article | Volume: 3, Issue: 10, October, 2013

Stability indicating chromatographic techniques for the determination of pipoxolan HCl

Hala E. Zaazaa Afaf O. Mohamed Mohamed Abdelkawy and Maha A.Hawwam   

Open Access   

Published:  Oct 31, 2013

DOI: 10.7324/JAPS.2013.31011

Two simple, accurate and sensitive methods were developed for the determination of pipoxolan HCl in presence of its degradation product. HPLC method (A), performed on C18 column using acetonitrile: 1mM ammonium acetate (80:20 v/v) as a mobile phase with a flow rate of 1.8ml/min. Detection was performed at 210 nm. TLC-densitometric method (B), using silica gel 60 F254 plates; the optimized mobile phase was chloroform: toluene: methanol: 10% ammonia (6:5:3:0.1 v/v). Quantitatively the spots were scanned densitometrically at 210 nm. Linearity ranges were 1-10 μg/ml for method A and 2-20 μg/band for method B, with mean percentage recoveries 99.38±0.672% and 99.32±0.97% for methods A and B, respectively. The proposed methods were found to be specific for pipoxolan HCl in presence of up to 90% of its degradation product. Statistical comparison between the results obtained by these methods and the manufacturer’s method was done,and it was found that there was no significant differences between them.

Keyword:     Pipoxolan HCl HPLC TLC-densitometric Stability.


Hala E. zaazaa, Afaf O. Mohamed, Mohamed Abdelkawy and Maha A. Hawwam. Stability–Indicating Chromatographic Techniques for the Determination of pipoxolan Hcl. J App Pharm Sci, 2013; 3 (10): 066-073.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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