Research Article | Volume: 3, Issue: 8, Supplement: 1, September, 2013

Histopathological and ultrastructural effects of methyl parathion on rat testis and protection by selenium

Mohamed SA. El-Gerbed   

Open Access   

Published:  Sep 19, 2013

DOI: 10.7324/JAPS.2013.38.S9

The present study was conducted to evaluate the possible protective effect of selenium in reversing methyl parathion -induced testicular damage in male rats, using light and electron microscopy with reference to plasma testosterone (T) and lutenizing hormone (LH) levels. The animals were randomly divided into four groups, Group 1: control animals. Group 2: animals were treated with sodium selenite in a dose (10µg/kg b.w). Group 3: rats were given methyl parathion in a dose (0.28 mg/kg b.w). Group 4: rats were received sodium selenite prior to treatment with methyl parathion. Results showed that animals exposed to methyl parathion displayed a reduction in body and testicular weight and a reduction in seminiferous tubules diameter. At the end of the treatments plasma testosterone and LH concentrations were reduced significantly in methyl parathion-treated rats. Light and electron microscopy of testes from treated animals with methyl parathion showed disorganization of tubular elements with increased intercellular space. Also, revealed necrosis and cellular damage. In addition, a significant increase in sperm DNA fragmentation was detected. Selenium supplementation to rats given methyl parathion improved the testicular damage. In conclusion, Selenium may be useful for the prevention and treatment of methyl parathion -induced testicular damage.

Keyword:     Methyl parathion Selenium Testicular damage TUNEL


Mohamed SA. El-Gerbed., Histopathological and ultrastructural effects of methyl parathion on rat testis and protection by selenium. J App Pharm Sci, 2013; 3 (8 Suppl 1): S53-S63.

Copyright:The Author(s). This is an open access article distributed under the Creative Commons Attribution Non-Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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