Abstracting and Indexing

Scopus, Chemical Abstracts, CAB abstracts, Hinari, Global Health, EBSCO Publishing's Electronic Databases, Summon by serial solutions, Abstracts on Hygiene and Communicable Diseases, Proquest, Tropical Diseases Bulletin, Open j-Gate, Google Scholar, Science Central, Ulrich's Periodicals Directory, Scimago Journal Ranking, AYUSH Research Portal, Geneva Foundation for Medical Education and Research, ABC Chemistry, Biblioteca, Necker, Academic Journals Database, InfoTrac Custom & Academic Onefile (Gale), Index Medicus for South-East Asia Region (IMSEAR), CNKI scholar.

News & Events

  • JAPS is approved by UGC.
  • JAPS has implemented the CrossMark, a multi-publisher initiative from CrossRef for forthcoming articles [Read more]
  • All the contents published in JAPS are archived in Portico, which provides permanent digital archiving for scholarly journals.
  • COPE Guidelines for Peer Reviewers.
  • Reviewers invited: Eminent professionals are invited to join JAPS reviewer panel. Interested candidates may send their applications to editor@japsonline.com

Home > Past Issue

Journal of Applied Pharmaceutical Science Volume: 3, Issue: 6, June, 2013
DOI: 10.7324/JAPS.2013.3617
ISSN 2231-3354

Research Article

Design of dissolution media for in-vitro bioequivalence testing of Lamivudine

Nagiat T Hwisa, Shanta Kumari Adiki, Prakash Katakam and Babu Rao Chandu

[Download PDF]


The present investigation is aimed at developing the stability indicating dissolution media for the determination of lamivudine (3TC) in pharmaceutical dosage forms. The stability of 3TC was tested in various dissolution media maintained at ambient temperature and 37 oC for 48 hrs. Stability studies of 3TC in various media indicated that the drug was stable in 0.1M HCl, pH 1.2 KCl-HCl buffer, pH 6.2 and pH 7.0 phosphate buffers. The λmax were found to be 280.0, 278.8, 273.0 and 271.5nm for 0.1M HCl, pH 1.2 KCl-HCl buffer, pH 6.2 and pH 7.0 phosphate buffers respectively with low CV of < 4.44%. The linearity of standard plots in optimized media was 0.5-40 µg/ml for 0.1M HCl and pH 1.2 KCl-HCl buffer. Similarly it was 0.5-60 µg/ml in pH 6.2 buffer and 0.2-40 µg/ml in pH 7.0 phosphate buffer. The validated methods were applied to determine 3TC concentration in formulations. In-vitro dissolution testing indicated that the 3TC was stable and drug release is uniform from tablet dosage forms. The optimized media could be employed to study the dissolution profiles of 3TC in bioequivalence studies.

Keywords: Lamivudine, Stability indicating, Bioequivalence studies, Dissolution media.