Activity of inhibit the cell cycle and induct apoptosis in HeLa cancer cell with combination of Sabrang onion (Eleutherine palmifolia (L.) Merr) and Starfruit Mistletoe (Macrosolen cochinchinensis (Lour.) Tiegh)

Roihatul Mutiah; Anik Listiyana; Arief Suryadinata; Rahmi Annisa; Abdul Hakim; Wirda Anggraini; Retno Susilowati

doi:10.7324/JAPS.2018.81016

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Abstract

The combination of Eleutherine palmifolia (L.) Merr (EP) and Macrosolen cochinchinensis (Lour.) Tiegh (MC) extracts has been proven an empirically potential cure for cancer. The aims of this research were to prove scientifically the anticancer potential of the combination of EP and MC as well as its effects on the cell cycle and the apoptosis of HeLa cancer cell. The MTT method was used to measure cytotoxic effects. The effect of inhibition of cell cycle and induction of cell apoptosis was measured by the flow cytometry method. The results showed that the combination of EP and MC gives an induction of apoptosis effect of HeLa cancer cell through inhibition of cell cycle in the G0-G1, S, and G2/M phases synergistically until it is synergist strong. Therefore, the combination of EP and MC can be recommended as a candidate drug for cancer therapy.

Keyword: Eleutherine palmifolia (L.) Merr Macrosolen cochinchinensis (Lour.) Tiegh anti-cancer HeLa cervical cancer cell.

Citation:

Mutiah R, Anik L, Arief S, Annisa R, Hakim A, Anggraini W, Susilowati R. Activity of inhibit of the cell cycle and induct apoptosis in HeLa cancer cell with combination of Sabrang onion (Eleutherine palmifolia (L.) Merr) and Starfruit Mistletoe Macrosolen cochinchinensis (Lour.) Tiegh extracts. J App Pharm Sci, 2018; 8(10): 122-128.

Copyright: © The Author(s). This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Mutiah R [PubMed] [Google Scholar ]

Listiyana A [PubMed] [Google Scholar ]

Suryadinata A [PubMed] [Google Scholar ]

Annisa R [PubMed] [Google Scholar ]

Hakim A [PubMed] [Google Scholar ]

Anggraini W [PubMed] [Google Scholar ]

Susilowati R [PubMed] [Google Scholar ]

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Arruebo M, Vilaboa N, Gutierrez BS, Lambea J, Tres A, Valladares M, Farnandez AG. Assessment of the evolution of cancer treatment therapies. Cancers, 2011; 3:3279–330.https://doi.org/10.3390/cancers3033279

Febrinda AE, Yuliana ND, Ridwan E, Wresdiyati T, Astawan M. Hyperglycemic control and diabetes complication preventive activities of Bawang Dayak (Eleutherine palmifolia L. Merr.) bulbs extracts in alloxan-diabetic rats. Int Food Res J, 2014; 21:1405–11.

Fitri Y, Suwarso E. Effects of inhibition cell cycle and apoptosis of sabrang onion extract (Eleutherine bulbosa (Mill.) Urb.) on breast cancer cells. Int J PharmTech Res, 2014; 6:1392–96.

Gibellini L, Pinti M, Nasi M, Montagna JP, De Biasi S, Cooper EL, Cossarizza A. Quercetin and cancer chemoprevention. Evid Based Complement Alternat Med, 2011; 201:1–15.https://doi.org/10.1093/ecam/neq053

Hou C, Li W, Li Z, Gao J, Chen Z, Zhao X, Yang Y, Zhang X, Song Y. Synthetic isoliquiritigenin inhibits human tongue squamous carcinoma cells through its antioxidant mechanism. Hindawi Oxid Med Cell Longev, 2017; 1:1–11.https://doi.org/10.1155/2017/1379430

Iwashita K, Kobori M, Yamaki K, Tsushida T. Flavonoids inhibit cell growth and induce apoptosis in B16 Melanoma 4A5 cells. Biosci Biotchnol Biochem, 2000; 64:1813–20.https://doi.org/10.1271/bbb.64.1813

Moitra K. Overcoming multidrug resistance in cancer stem cells. BioMed Res Int, 2015; 1:1–8.https://doi.org/10.1155/2015/635745

Mutiah R. Pengembangan Fitofarmaka Antikanker Panduan Teknik Pengembangan Obat Herbal Indonesia Menjadi Fitofarmaka. Uin Maliki Press, 2014. ISBN: 978-602-1190-26-5 [Indonesian].

Mutiah R, Listiyana A, Indradmojo C, Griana TP, Dwi HH, Atmaja RR. Induction of apoptosis and phase-cell cycle inhibition of G0- G1, S, G2-M of T47D breast cancer cells on treatment with ethyl acetate fraction of jackfruit parasite leaves (Macrosolen cochinchinensis). J Appl Pharma Sci, 2017; 7:138–43.

Mutiah R, Widyawaruyanti A, Sukardiman S. Calotropis gigantea leaf extract increases the efficacy of 5-fluorouracil and decreases the efficacy of doxorubicin in Widr colon cancer cell culture. J Appl Pharm Sci, 2018a; 8:51–6.

Mutiah R, Widyawaruyanti A, Sukardiman S. Calotroposid A: a glycosides terpenoids from calotropis gigantea induces apoptosis of colon cancer WiDr cells through cell cycle arrest G2/M and caspase 8 expression. Asian Pac J Cancer Prev, 2018b; 19:1457–64.

Rahman MM, Chowdhury MAU, Uddin ME, Islam AMT, Hossain MA. Macrosolen cochinchinensis (Lour.): anti-nociceptive and antioxidant activity. Asian Pac J Trop Biomed, 2012; 2:203–7.https://doi.org/10.1016/S2221-1691(12)60160-9

Reynolds CP, Maurer BJ. Evaluating response to antineoplastic drug combinations in tissue culture models. Methods Mol Med, 2005; 110: 173–83.https://doi.org/10.1385/1-59259-869-2:173

Tacar O, Sriamornsak P, Dass CR. Doxorubicin: an update on anticancer molecular action, toxicity and novel drug delivery systems. J Pharm Pharmacol, 2013; 65:157–70.https://doi.org/10.1111/j.2042-7158.2012.01567.x

Takahashi T, Takasuka N, Ligo M, Baba M, Nishino H, Tsuda H, Okuyama T. Isoliquiritigenin, a flavonoid from licorice, reduce prostaglandin E2 and nitric oxide, causes apoptosis, and suppress aberrant crypt foci development. Cancer Sci, 2004; 95:448–53.https://doi.org/10.1111/j.1349-7006.2004.tb03230.x

Torre L, Bray F, Siegel R, Ferlay J, Lorte T, Jemal A. Global cancer statistics. CA Cancer J Clin, 2015; 62:87–108.https://doi.org/10.3322/caac.21262

Wang Z, Wang N, Han S, Wang D, Mo S, Yu L, et al. Dietary compound isoliquiritigenin inhibits breast cancer neoangiogenesis via VEGF/VEGFR-2 signaling pathway. PLoS One, 2013; 8:1–14.https://doi.org/10.1371/journal.pone.0068566

World Health Organization (WHO). WHO guidance note: comprehensive cervical cancer prevention and control: a healthier future for girls and women. WHO Press Geneva, Switzerland, 2013.

Worthley DL, Whitehall VL, Spring KJ, Leggett BA. Colorectal carcinogenesis: road maps to cancer. World J Gastroenterol, 2007; 13: 3784–91.https://doi.org/10.3748/wjg.v13.i28.3784

Xiao X, Shi D, Liu L, Wang J, Xie X, Kang T, Deng W. Quercetin suppresses cyclooxygenase-2 expression and angiogenesis through inactivation of P300 signaling. PLoS One, 2011; 6:1–10.https://doi.org/10.1371/journal.pone.0022934

Yuan H, Ma Q, Ye L, Piao G. The traditional medicine and modern medicine from natural products. Molecules, 2016; 21:1–18.https://doi.org/10.3390/molecules21050559

Yusni MA, Metria IB, Budiani DR, Mudigdo A. Perbedaan Pengaruh Pemberian Fraksi Etanolik Bawang dayak (Eleutherine palmifolia L.Merr) dengan 5-Fluorouracil Terhadap Penghambatan Pertumbuhan Galur Sel Karsinoma Kolon HT29 dan Ekpresi p53 Mutan. Medical Faculty Universitas Sebelas Maret, 2008; 65–76 [Indonesian].